|
KMID : 0380020120270030186
|
|
Korean Journal of Biotechnology and Bioengineering
2012 Volume.27 No. 3 p.186 ~ p.194
|
|
|
Characterization of HEK293 and Namalwa Cell Cultures by Using Design of Experiment
|
|
Kang Kyung-Ho
Seo Joon-Serk
Kim Dong-Il
|
|
|
Abstract
|
|
|
|
Various human host cell lines, which are more effective than the other original human cell lines, have been developed and used. Highly efficient human cell line can be obtained from the fusion between human embryonic kidney 293 (HEK293) and human Burkitt's lymphoma cells (Namalwa). Fused cell line has the advantages of both cell lines such as the high transfection efficacy of HEK293 cells and the constitutive expression of Epstein-Barr virus (EBV) genome which is related with high expression of target protein and anti-apoptotic growth of Namalwa cells. In this study, characterization of two original cell lines was performed by using design of experiment (DOE) considering cell maintenance, media development, optimization of culture condition, and scale-up. The formation of aggregates was apparent with high glutamine concentration at more than 6 mM. Supplementation of hydrolysates showed positive effects on the growth performances of HEK293 cells. On the contrary, Namalwa cells showed negative results. It was confirmed that Namalwa cells were more sensitive to lower temperature at 35oC and hyperosmotic condition over 260 mOsm/kg. In addition, both cell lines showed limited growth in 3-L bioreactor due to shear stress.
|
|
|
KEYWORD
|
|
|
Human cell line, HEK293, Namalwa, design of experiment
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|